next generation growth medium

mediabottles2ACSelerateMAX Xeno-Free hMSC Growth Medium is a complete media formulation originally created to support the rapid production of large numbers of multipotent adipose tissue-derived MSCs. ACSelerateMAX has also been extensively documented to support the robust expansion of human MSCs derived from bone marrow, umbilical cord, placenta, and urine.

The expansion of human “adult” mesenchymal stem cells (MSCs) in the presence of Fetal Bovine Serum (FBS) presents numerous problems. These may include undesirable epigenetic programming by non-human serum proteins which may lead to inappropriate lineage commitments as well as perturbations and clouding of experimental results. Experimental results obtained when using FBS may also not translate directly or quickly into meaningful applications in the human therapeutic or regenerative medical fields due to the need to replicate experimental results under xeno-free conditions. To circumvent these issues and to support the pursuit of more realistic, more easily translatable experimental results, American CryoStem developed ACSelerateMAX.

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mediabottle-3carefully crafted ingredients

The combination of ingredients in ACSelerateMAXTM has been crafted to mimic the chemical environment of the perivascular niche that surrounds blood vessels, where MSCs are often located. This includes a modest human serum component and a cocktail of specific recombinant growth factors known to drive MSC proliferation. By providing a similar chemical environment to what cells in the perivascular space would encounter, more natural results would be expected.

Routine cellular doubling times of between 11 and 30 hours are common, and expansions of >25 total doublings (>33 million-fold) are also common, with expansions of over 30 doublings (>1 billion-fold) being attainable. The immunophenotypes as well as the multipotent differentiation potentials of these expanded cells have been documented to be maintained past 30 doublings in multiple cell lines.


ACSelerate-MAXTM Mesenchymal Stem Cell Growth Medium has been shown to support the expansions of mesenchymal stem cells (MSCs) derived from human umbilical cord (not shown), human bone marrow, human placenta, human adipose tissue, and human urine (not shown). The phenotypic identity of the cells derived from umbilical cord, bone marrow, placenta, and adipose tissue has been shown to be CD14-, CD19-, CD29+, CD31-, CD34-, CD44+, CD45-, CD49D+, CD73+, CD90+, CD105+, and CD146+, defining them as MSCs, and full multipotency has been shown at greater than 30 doublings. Urine-derived MSCs, by contrast, show the phenotype of CD14-, CD19-, CD29+, CD31-, CD34-, CD44+ CD45-, CD49D+, CD73Low/-, CD90+/-, CD105-, CD146+.


Fluorescent immunostaining of adipose tissue-derived mesenchymal stem cells (ADMSCs) grown in ACSelereateMAXTM hMSC Medium.


Fluorescent immunostaining of bone marrow-derived mesenchymal stem cells (ADMSCs) grown in ACSelereateMAXTM hMSC Medium.


Fluorescent immunostaining of placental-derived mesenchymal stem cells (ADMSCs) grown in ACSelereateMAXTM hMSC Medium.