Differentiation Capabilities of ADSCs

Our proprietary ATCELLs combined with our differentiation medium technology creates defined cell types:


Differentiation is the term used to describe the ability to transition a stem cell into another cell type such as cartilage or bone cells. ADSC’s have a natural tendency to change into bone, cartilage, muscle, fat and to other types of cells. As part of our commitment to continually improve our stem cell differentiation capabilities our internal R&D development program is focused on optimizing the full capabilities of clients adipose derived stem cells. American CryoStem has created a line of patented medium products, ACSelerate CY (cartilage), ACSelerate OB (bone) and ACSelerate AD (fat) that can successfully transform an ADSC into cartilage, bone and fat cells. CRYO is currently designing and testing new formulations for muscle and nerve cells.

All interested parties should email info@americancryostem.com to learn more about our stem cell expansion and differentiation capabilities.

ACSelerate™ AD is a defined, animal product-free patented medium intended to drive the differentiation of adipose-derived stem cells (ADSCs) to adipocyte (fat cell)  lineage. This cellular change may be ascertained by the visible formation of intracellular oil droplets which become larger and more numerous (Below Left).  The identity of the droplets has been positively verified by staining with Oil Red (Below Right):

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ACSelerate™ CY is a defined, animal product-free patented medium intended to drive the differentiation of adipose-derived stem cells (ADSCs) to the chondrocytes (cartilage)  lineage.  Early differentiation may be identified by changes in characteristics from elongated, fibroblast like cells to rounded “cobblestone” clumping of cells into chondrogenic foci  (Below Left).  Later stage chondrogenic differentiation has been confirmed by staining with Alcian Blue (Below Right):

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ACSelerate™ OB is a defined, animal product-free patented medium intended to drive the differentiation of adipose-derived stem cells (ADSCs) to osteocyte (bone) lineage.  Commitment to this lineage is identified by pronounced, visible mineralization (Below Left) which becomes more pronounced over time.  Positive confirmation has been obtained by staining with Alizarin Red (Below Right).

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